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Image Search Results
Journal: PLoS ONE
Article Title: Notch Signaling Regulates Bile Duct Morphogenesis in Mice
doi: 10.1371/journal.pone.0001851
Figure Lengend Snippet: A,B. Hematoxylin and eosin-stained sections at P7 of control littermate (CT) and Notch2-cko mice using the Notch2 del2 allele. Bile ducts (arrow) are observed in the periportal region of the control littermate (A), but not the Notch2-cko mouse (B). C–F. DBA lectin staining. C,D. Control littermate and Notch2-cko mice using the Notch2 del2 allele at P7. E,F. Control littermate and Notch2-cko mice using the Notch2 del3 allele at P3. DBA-positive cells form patent bile ducts (arrows) adjacent to the portal veins in control mice (C,E). In Notch2-cko mice using either the Notch2 del2 (D) or Notch2 del3 (F) allele, small numbers of DBA-positive cells (arrowheads) are present adjacent to the portal veins, but these cells have not formed patent ducts. G,H. Cytokeratin immunostaining of control littermate and Notch2-cko mice ( Notch2 del2 allele) at P7. The ductal plate of the control liver (G) has remodeled into epithelial bile ducts (arrows), while the Notch2-cko liver (H) exhibits only ductal plate remnants (arrowheads).
Article Snippet: The antibodies and lectins used in these studies were rabbit polyclonal anti-human cytokeratins (Dako, Cat. A0575); rabbit polyclonal anti-HNF1β (Santa Cruz, Cat. sc-22840); rabbit polyclonal anti-HNF6 (Santa Cruz, Cat. sc-13050); and
Techniques: Staining, Immunostaining
Journal: Frontiers in Oncology
Article Title: Multi-Faceted Effects of ST6Gal1 Expression on Precursor B-Lineage Acute Lymphoblastic Leukemia
doi: 10.3389/fonc.2022.828041
Figure Lengend Snippet: BCP-ALL cells contain high levels of α2-6 sialylation. (A) Western blot of different BCP-ALL cell lines probed with SNA lectin to specifically detect α2-6-linked sialic acids on glycoproteins. GAPDH, loading control. Location of molecular weight standards to the right. (B) Analysis of N-glycans in RS4;11 cells as previously described . Combined results of 15 individual RS4;11 cell samples. Overall, more than 65% of all identified N-glycans were found to be sialylated with 7.4% in α2-3, 14.1% in α2-3/6, and 45.1% in α2-6 attachment.
Article Snippet: BCP-ALL cells were lysed in Triton T-100 lysis buffer with glycerol at pH 7.4 (Alfa Aesar, Cat#J63866AK) and glycoproteins were captured with
Techniques: Western Blot, Molecular Weight